Recombinant Human CD3D&CD3E Heterodimer (C-Fc-Flag&C-Fc-6His)

• Background:

  T-cell surface glycoprotein CD3D & CD3E, also known as CD3 delta & CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

• Abbreviation:

  CD3D&CD3E Heterodimer

• Synonyms:

  CD3 delta & CD3 epsilon; CD3 delta / epsilon; CD3E & CD3D

• UniProt:

  P04234; P07766

• Accession Number:

  P04234&P07766

• Expression System:

  HEK293 Cells

• Tag:

  C-Fc-Flag & C-Fc-His

• Sequence:

  Phe22-Ala105&Asp23-Asp126

• Field of Research:

  Immunology; Stem cells

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  37 & 39.2 kDa

• Observed Molecular Weight:

  40 - 60 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human