Recombinant Human/Cynomolyus CD28/TP44 (C-Fc-Avi) Biotinylated

• Background:

  T-cell-specific surface glycoprotein CD28 (CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin (Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) . When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs) . The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

• Abbreviation:

  CD28, TP44

• Synonyms:

  CD28; CD28 antigen; CD28 molecule; T-cell-specific surface glycoprotein CD28; TP44; Tp44

• UniProt:

  P10747

• Accession Number:

  P10747

• Expression System:

  HEK293 Cells

• Conjugation:

  Biotinylated

• Tag:

  C-Fc-Avi

• Sequence:

  Asn19-Pro152

• Applications:

  BLI

• Field of Research:

  Immunology; Stem cells

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Loaded Human B7-1-His on HIS1K Biosensor, can bind Biotinylated Human/Cynomolgus CD28-Fc-Avi with an affinity constant of 9.09 pM as determined in BLI assay.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  44.1 kDa

• Observed Molecular Weight:

  60 - 90 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mTris-HCl, 150mM NaCl, pH 8.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human, Cynomolyus