Recombinant Human Siglec-10 (C-Fc)

• Background:

  Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

• Abbreviation:

  Siglec-10

• Synonyms:

  MGC126774; PRO940; SIGLEC10; SLG2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7; Siglec-10; Siglec-like protein 2; Siglec10; sialic acid-binding Ig-like lectin 10; siglec-like gene 2

• UniProt:

  Q96LC7

• Accession Number:

  Q96LC7

• Expression System:

  HEK293 Cells

• Tag:

  C-Fc

• Sequence:

  Met17-Thr546

• Applications:

  ELISA

• Field of Research:

  Tags & Cell Markers

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Immobilized Anti-Human Siglec10 mAb at 2μg/ml (100 μl/well) can bind Human Siglec-10-Fc. The ED50 of Human Siglec-10-Fc is 9.18 ng/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  85.1 kDa

• Observed Molecular Weight:

  110 - 120 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM Tris-HCl, 6% Sucrose, 4% Mannitol, 50mM NaCl, 0.05% Tween 80, pH 8.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human