Recombinant Human Leukocyte Ig-Like Receptor B2/LILRB2/ILT4/CD85d (C-Fc)

• Background:

  Members of the immunoglobulin-like transcript (ILT) family are activating and inhibitory immunoreceptors whose genes are located same locus that encodes killer cell Ig-like receptors (KIR) . Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2 (LIR-2) is a type I transmembrane protein. LIR-2 is expressed primarily on monocytes and dendritic cells (DC) . Human LIR-2 is produced as a 598 amino acino acid precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmenbrane segment, and a 116 aa cytoplasmic domain. LIR-2 binds to Classical MHCI proteins. Ligation of LIR-2 incluces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1. LIR-2 mediates tolerogenic DC-induced CD4+ T cell energy in vitro and in vivo.

• Abbreviation:

  LILRB2, ILT4, CD85d

• Synonyms:

  CD85 Antigen-Like Family Member D; CD85d; ILT; ILT-4; Immunoglobulin-Like Transcript 4; LILRB2; LIR-2; Leukocyte Immunoglobulin-Like Receptor 2; Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2; MIR-10; Monocyte/Macrophage Immunoglobulin-Like Receptor 10

• Accession Number:

  AAH36827.1

• Expression System:

  HEK293 Cells

• Tag:

  C-Fc

• Sequence:

  Gln22-His458

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  74.5 kDa

• Observed Molecular Weight:

  90 - 120 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human