Recombinant Human CD28/TP44 Protein (Fc Tag)

• Background: T-cell-specific surface glycoprotein CD28 (CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin (Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals; which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival; interleukin-2 production; and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) . When activated by Toll-like receptor ligands; the CD80 expression is upregulated in antigen presenting cells (APCs) . The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
• Abbreviation: CD28, TP44
• Synonyms: CD28; CD28 antigen; CD28 molecule; T-cell-specific surface glycoprotein CD28; TP44; Tp44
• UniProt: P10747
• Accession Number: P10747
• Expression System: HEK293 Cells
• Tag: C-Fc
• Sequence: Asn19-Pro152
• Field of Research: Immunology; Stem cells
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: Please refer to the printed manual for detailed information.
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Calculated Molecular Weight: 42.3 kDa
• Observed Molecular Weight: 60 - 80 kDa
• Formulation: Lyophilized from a 0.2 µm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.
• Species: Human