Recombinant Human CCL2/MCP-1 Protein

• Background:

  The chemokine (C-C motif) ligand 2 (CCL2), also known as monocyte chemoattractant protein (MCP) -1 and small inducible cytokine A2 (SCYA2) ), is a small cytokine that belongs to the CC chemokine family responsible for monocyte attraction. Its cognate receptor, CCR2, play a critical role in regulating nociceptive processes during neuropathic pain. Both CCL2 and CCR2 are implicated in induction of autoimmunity. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. Recently research also showed that CCL2 might be useful as a biomarker of fibrosis as well as a target for therapeutic intervention.

• Abbreviation:

  CCL2, MCP-1

• Synonyms:

  C-C motif chemokine 2; CCL2; HC11; MCAF; MCP-1; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Small-inducible cytokine A2

• UniProt:

  P13500

• Accession Number:

  P13500

• Expression System:

  E. coli

• Tag:

  N-His

• Sequence:

  Gln24-Thr99

• Applications:

  Cell culture

• Field of Research:

  Cardiovascular; Cancer; immunology; metabolism

• Endotoxin:

  < 0.1 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measure by its ability to chemoattract BaF3 cells transfected with human CCR2A.The ED₅₀ for this effect is <20 ng/mL.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  9.5 kDa

• Observed Molecular Weight:

  11 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human