Recombinant Human SELP/selectin P/P-selectin Protein (Fc Tag)

• Background:

  P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.

• Synonyms:

  CD62; CD62P; GMP140; GRMP; LECAM3; P-Selectin; PADGEM; PSEL

• UniProt:

  P16109

• Accession Number:

  AAN06828.1

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Sequence:

  Met 1-Ala 771

• Applications:

  Cell culture

• Field of Research:

  Cardiovascular, Immunology, Stem Cells

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10⁴ cells/well are added to SELP/Fc Chimera coated plates (10 μg/mL with 100 μL/well), > 80% cells will adhere after 1 hour at 37°C.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  107 kDa

• Observed Molecular Weight:

  130 - 150 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human