Biotinylated Rhesus macaque CD155 / PVR Protein, His,Avitag™ (MALS verified)

Biotinylated Rhesus macaque CD155 / PVR Protein, His,Avitag™ (MALS verified)

• Background: CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains. CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.
• Description: Biotinylated Rhesus macaque CD155 Protein, His,Avitag (CD5-R82E4) is expressed from human 293 cells (HEK293). It contains AA Asp 28 - Asn 343 (Accession # Q0MSE6-1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 38.0 kDa. The protein migrates as 45-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Asp 28 - Asn 343
• Host: HEK 293
• Target: CD155
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Rhesus macaque
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Biotinylated Rhesus macaque CD155 Protein, His,Avitag (Cat. No. CD5-R82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Cynomolgus / Rhesus macaque TIGIT, Mouse IgG2a Fc Tag (Cat. No. TIT-C5253) with a linear range of 0.1-2 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 38.0 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C