Hematoxylin, PROBE

• Description:

  This uniquely formulated Probe hematoxylin is an excellent counterstain for in situ DNA hybridization procedure.

• Specifications:

  Ready to use counterstain for in situ hybridization

• Other Statements:

  For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

• Label:

  ICT

• Type:

  IHC Reagents

• Applications:

  IHC

• Assay Protocol:

  1. After chromogen staining, wash slide 2-3X with de/di water, 2X with 2. Apply Probe hematoxylin for 2-3 minutes; wash with tap water 5-7X. 3. Soak the slides in Immuno Automation buffer (ImmunoBioScience AR-6561), PBS or any buffer with pH 7.4-8 for 2-3 minutes. Hematoxylin changes color from purple red to blue. 4. Wash slide 2-3X with de/di water. 5. The slides can be mounted with aqueous mounting medium (ImmunoHistoMount (AR-6503) or equivalent) for chromogens like AEC, DAB, Fast red, BCIP/INT, or Super Fast red. DAB and Super Fast can be mounted with Organic mounting mediums like Organo mount / limonene (AR-6504) or equivalent after dehydrating the tissue with alcohols and organic solvents.

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Temperature:

  Room temperature

• Target Description:

  This uniquely formulated Probe hematoxylin is an excellent counterstain for in situ DNA hybridization procedure. Probe hematoxylin produces clean blue color of nuclei on formalin-fixed paraffin-embedded (FFPE) tissue sections and frozen sections and is stable for 2-3 years when kept in the dark. Probe hematoxylin will produce a blue nuclear counterstain which is lighter than conventional hematoxylin counterstain. The counterstain can be used with HRP chromogens, AEC, DAB and alkaline phosphatase chromogens, fast red, BCIP/INT and super fast red (ImmunoBioScience). Sides stained with this hematoxylin can be mounted with aqueous or organic mounting mediums.