Mouse Anti-Mouse VEGF-A, Blocking Antibody Monoclonal Antibody

Mouse Anti-Mouse VEGF-A, Blocking Antibody Monoclonal Antibody

• Description: Mouse VEGF (or VEGF-A) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant murine VEGF (isoform 164) is a 39.0 kDa disulfide-linked homodimeric protein consisting of two 164 amino acid polypeptide chains. The antibody blocks the binding of VEGF to VEGFR-2 ( mouse FLK) and therefore the initiation of signal transduction.
• Synonyms: Vegfa; Vpf; Vegf; Vegf120; Vegf164; Vegf188
• CAS Number: 9007-83-4
• NCBI Gene ID: 22339
• UniProt: Q00731
• Accession Number: NP_001020421
• Accession Number mRNA: NM_001025250
• Gene Location: 17C; 17 24.2cM
• Host: Mouse
• Origin Species: Mouse
• Species Reactivity: Mouse
• Target Antigen: Recombinant Mouse VEGF
• Isotype: IgG1
• Clone: (#MAB0705)
• Validated Applications: N, WB
• Purification Method: Protein G chromatography
• Detection Range: WB (mouse/human); Neutralization of VEGF: human, mouse
• Form: Lyophilized
• Buffer: PBS
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute the antibody with 200 µl sterile PBS and the final concentration is 500 µg/ml.
• Shipping Conditions: Room Temperature
• Storage Conditions: Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.