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Caspase-8 Substrate IETD-AFC

CAT:
26-1062-1000
Size:
1000 Assays
Price:
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For price, please contact [email protected]
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
Caspase-8 Substrate IETD-AFC - image 1

Caspase-8 Substrate IETD-AFC

  • Description: Ready-to-use fluorometric substrate for FLICE/caspase-8 and related caspases that recognize the amino acid sequence IETD. The sequence IETD is based on caspase-8 cleavage site in CPP32/caspase-3 proenzyme. FLICE and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate IETD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
  • Shipping Conditions: gel pack
  • Storage Conditions: -20°C
  • Shelf Life: 12 months