Mouse Siglec-3 / CD33 Protein, His Tag

• Background:

  Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

• Description:

  Mouse Siglec-3, His Tag (CD3-M52H4) is expressed from human 293 cells (HEK293) . It contains AA Asp 18 - Glu 240 (Accession # Q63994-1) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  Q63994-1

• Expression Region:

  Asp 18 - Glu 240

• Host:

  HEK293

• Target:

  Siglec-3

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  Mouse

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  26.5 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Mouse