Mouse CTLA4:B7-1 TR-FRET Assay

• Description:

  The mouse CTLA4:B7-1 TR-FRET Assay is designed to measure the inhibition of mouse CTLA4 binding to mouse B7-1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated mCTLA4, mB7-1 protein, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.Need us to run inhibitor screens or profile your compounds against Mouse CTLA4:B7-1? Check out ourImmunotherapy Biochemical Screening Services.

• Format:

  Catalog #ComponentAmountStorage79001mCTLA4, biotinylated*15 µg-80°CAvoid freeze/ thaw cycles!79002mB7-1-Flag-Avi-His*5 µg-80°C30017Anti-His Tb Donor2 x 10 µl-20°CDye-labeled Acceptor2 x 10 µl-20°C793113x Immuno Buffer 14 ml-20°C79969White, non-binding, low volume, 384-well microtiter plate1Room Temp*The initial concentrations of mCTLA4 and mB7-1 are lot-specific and will be indicated on the tubes containing the protein.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months from date of receipt when stored as directed.