Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody

• Description:

  Boster Bio Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody catalog # M03998. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Dual specificity mitogen-activated protein kinase kinase 5;MAP kinase kinase 5;MAPKK 5;2.7.12.2;MAPK/ERK kinase 5;MEK 5;MAP2K5;MEK5, MKK5, PRKMK5;

• CAS Number:

  9007-83-4

• Gene Name:

  Dual specificity mitogen-activated protein kinase kinase 5

• UniProt:

  P11177

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Immunogen:

  A synthesized peptide derived from human Pyruvate Dehydrogenase E1 beta subunit The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2) . It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) .

• Clonality:

  Monoclonal

• Clone:

  Clone: AEAD-16

• Tissue Specificity:

  Expressed in many adult tissues. Abundant in heart and skeletal muscle.

• Applications:

  WB,IHC,ICC,IF,IP,Flow Cytometry

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. .

• Components:

  Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

• Storage Conditions:

  Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  50112 MW

• Observed Molecular Weight:

  85 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:1000-1:5000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  IP 1:50
  FC 1:50

• Subcellular Location:

  Cytoplasm, cytoskeleton . Cell projection, lamellipodium . At the interface between the lamellipodial actin meshwork and the membrane. .

• Protein Name:

  T-cell surface glycoprotein CD3 zeta chain