Human Integrin alpha 1 beta 1 (ITGA1&ITGB1) Heterodimer Protein, His Tag&Tag Free

• Background:

  Integrin alpha-1/beta-1 is a receptor for laminin and collagen. The Integrin alpha-1/beta-1 and Integrin alpha-2/beta-1 integrins provide critical support for vascular endothelial growth factor signaling, endothelial cell migration, and tumor angiogenesis. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.

• Description:

  Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52Wb) is expressed from human 293 cells (HEK293) . It contains AA Phe 29 - Pro 1141 (ITGA1) & Gln 21 - Asp 728 (ITGB1) (Accession # P56199-1 (ITGA1) & P05556-1 (ITGB1) ) .

• Specifications:

  Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA1 and ITGB1, has a calculated MW of 130.0 kDa (ITGA1) and 83.7 kDa (ITGB1) . Subunit ITGA1 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 170-200 kDa (ITGA1) and 90-105 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P56199-1 (ITGA1) & P05556-1 (ITGB1)

• Expression Region:

  Phe 29 - Pro 1141 (ITGA1) & Gln 21 - Asp 728 (ITGB1)

• Host:

  HEK293

• Target:

  Integrin alpha 1 beta 1

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis | Native

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wb) with a linear range of 1-20 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  130.0 kDa (ITGA1) & 83.7 kDa (ITGB1)

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  500ug*2

• Species:

  Human