Recombinant Mouse CCL6/C10 Protein (Trx Tag)

• Background:

  In mice, CCL6/C10 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6/C10 is greatly reduced.CCL6/C10 can also be induced in the Mouse lung by the cytokine interleukin 13

• Abbreviation:

  CCL6/C10

• Synonyms:

  Scya; Chemokine C C motif Ligand; Small Inducible Cytokine A; chemokine (C-C motif) ligand; CCL 6; chemokine (C-C motif) ligand 6; Chemokine C C motif Ligand 6; MRP1; MRP-1; Small Inducible Cytokine A6; C10; Scya6; Ccl6; C10; CCL 6; chemokine (C-C motif) ligand 6; Chemokine C C motif Ligand 6; MRP1; SCYA6; Small Inducible Cytokine A6

• UniProt:

  P27784

• Accession Number:

  P27784

• Expression System:

  E. coli

• Tag:

  N-Trx

• Sequence:

  Gly22-Ala116

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  30.3 kDa

• Observed Molecular Weight:

  32 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Mouse