Rabbit anti Human Neurogenin-1

• Background:

  Acts as a transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. Efficient DNA binding requires dimerization with another bHLH protein. Expression restricted to the embryonic nervous system.

• Synonyms:

  Class A basic helix-loop-helix Protein 6, Neurogenic basic-helix-loop-helix Protein, Neurogenic differentiation factor 3, NeuroD3, bHLHa6, NGN-1

• CAS Number:

  9007-83-4

• UniProt:

  Q92886

• Host:

  Rabbit

• Species Reactivity:

  Human

• Isotype:

  IgG

• Conjugation:

  Affinity Purified

• Type:

  Antigen Immunoaffinity Purified Polyclonal

• Applications:

  WB, Enzyme Immunoassay, IHC

• Field of Research:

  Neuroscience

• Purification Method:

  Antigen Immunoaffiinity Purification

• Assay Principle:

  Antibody can be used for immunohistochemical staining (1-5 µg/mL). Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Velkey, J.M., et al. ‘Expression of Neurogenin 1 in Mouse Embryonic Stem Cells Directs the Differentiation of Neuronal Precursors and Identifies Unique Patterns of Down-stream Gene Expression.’ Dev. Dyn., 242, 230-253 (2013) 2. Hill, G.W., et al. ‘Netrin-1-mediated axon guidance in mouse embryonic stem cells overexpressing neurogenin-1.’ Stem Cells Dev., 21, 2827-2837 (2012) 3. Choi, C.I., et al. ‘Neural Induction with Neurogenin 1 Enhances the Therapeutic Potential of Mesenchymal Stem Cells in an ALS Mouse Model.’ Cell Transplant., Epub ahead of print (2012)

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  IHC