Recombinant Mouse IL7RA/CD127 Protein (Fc Tag)

• Background:

  Interleukin 7 Receptor alpha (IL-7Rα), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7Rα is majorly expressed on T cells and their precursors, and early in B cell development as well, prior to the appearance of surface IgM. Dynamic regulation of IL-7Rα is important for the generation of appropriate immune responses.

• Abbreviation:

  IL7RA, CD127

• Synonyms:

  CD127; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7RA; IL-7Ralpha; IL-7R-alpha; Interleukin-7 receptor subunit alpha; Il7r

• Accession Number:

  P16872

• Expression System:

  HEK293 Cells

• Tag:

  C-Fc

• Sequence:

  Glu21-Asp239

• Applications:

  BLI

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Loaded Human IL-7-His on HIS1K Biosensor, can bind Mouse IL-7RA-Fc with an affinity constant of 5.91 nM as determined in BLI assay.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  52.1 kDa

• Observed Molecular Weight:

  61 - 75 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM Tris-HCl, 10% Sucrose, 3% Glycine, 100mM NaCl, 0.1mM EDTA, 0.05% Tween 80, pH 8.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse