Recombinant Mouse TNF-alpha/TNFA Protein

• Background:

  Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.

• Abbreviation:

  TNF-alpha, TNFA

• Synonyms:

  Cachectin; DIF; Membrane Form; TNF-Alpha; TNF-a; TNF-alpha; TNFSF2; TNFalpha; Tnf; Tnfa; Tnfsf1a; Tnfsf2; Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2

• UniProt:

  P06804

• Accession Number:

  P06804

• Expression System:

  E. coli

• Tag:

  None

• Sequence:

  Asp89-Leu235

• Applications:

  Cell culture

• Endotoxin:

  < 0.01 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 2-8 pg/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  16.4 kDa

• Observed Molecular Weight:

  14 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse