Recombinant Mouse TGFB2 Protein

• Background:

  Transforming growth factor beta 2 (TGF-β2) is a member of TGF-beta superfamily that shares a characteristic cysteine knot structure. Mice with TGF-β2 gene deletion show defects in development of cardiac, lung, craniofacial, limb, spinal column, eye, inner ear and urogenital systems. All TGF-β isoforms signal via the same heteromeric receptor complex, consisting of a ligand binding TGF-β receptor type II (TβR-II), and a TGF-β receptor type I (TβR-I) . Signal transduction from the receptor to the nucleus is mediated via SMADs. TGF-β expression is found in cartilage, bone, teeth, muscle, heart, blood vessels, haematopoitic cells, lung, kidney, gut, liver, eye, ear, skin, and the nervous system.

• Abbreviation:

  TGFB2

• Synonyms:

  BSC-1 cell growth inhibitor; Cetermin; G-TSF; Glioblastoma-derived T-cell suppressor factor; MGC116892; Polyergin; TGF-beta-2; TGF-beta2; TGFB2; transforming growth factor beta-2

• UniProt:

  P27090

• Accession Number:

  P27090

• Expression System:

  HEK293 Cells

• Tag:

  None

• Sequence:

  Ala303-Ser414

• Applications:

  ELISA

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Immobilized Mouse TGFB2 at 10μg/ml (100 μl/well) can bind Human TGFBR2-FC (Cat: PKSH033426) . The ED₅₀ of Mouse TGFB2 is 0.136μg/mL.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  12.7 kDa

• Observed Molecular Weight:

  12 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 4mM HCl.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse