Recombinant Mouse c-MET/HGFR Protein (Fc Tag) (Fc Tag)

• Background:

  Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.

• Synonyms:

  AI838057; HGF; HGFR; Par4; c-Met

• Accession Number:

  NP_032617.2

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Sequence:

  Met 1-Asn 929

• Applications:

  ELISA

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 92 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Immobilized human HGF at 2 μg/ml (100 μl/well) can bind mouse HGFR with a linear ranger of 1. 28-32ng/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  32 & 96 kDa

• Observed Molecular Weight:

  43 & 115 - 120 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse