Recombinant Human NEDD8

• Background:

  Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

• Abbreviation:

  NEDD8

• Synonyms:

  NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; Ubiquitin-like protein Nedd8

• UniProt:

  Q15843

• Accession Number:

  Q15843

• Expression System:

  E. coli

• Tag:

  None

• Sequence:

  Met1-Gly76

• Field of Research:

  Cell biology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  8.6 kDa

• Observed Molecular Weight:

  9 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM Tris-HCl, 250mM NaCl, pH 8.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human