Recombinant Human Wnt3a/Wnt-3a

• Background:

  Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity) . Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) .

• Abbreviation:

  Wnt3a, Wnt-3a

• Synonyms:

  MGC119418; MGC119419; MGC119420; Wnt-3a; Wnt3a; member 3A; protein Wnt-3a; wingless-type MMTV integration site family

• Expression System:

  HEK293 Cells

• Tag:

  None

• Applications:

  Cell culture

• Endotoxin:

  < 0.01 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is 71.45 ng/ml.

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  58.5 kDa

• Observed Molecular Weight:

  66 - 73 kDa

• Formulation:

  Supplied as a 0.2 µm filtered solution of 25mM Tris-HCl, 500mM NaCl, pH8.2.

• Species:

  Human