Recombinant Human Cbl-c/CBL-3 Protein (His & GST Tag)

• Background:

  CBL proteins, such as Cbl-c, are phosphorylated upon activation of a variety of receptors that signal via protein tyrosine kinases. Through interactions with proteins containing SRC homology-2 (SH2) and SH3 domains, CBL proteins modulate downstream cell signaling. Cbl-c is a member of the Cbl family of E3 ubiquitin ligases. Expression of Cbl-c gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for Cbl-c gene.

• Synonyms:

  CBL-3; CBL-SL; RNF57

• UniProt:

  Q9ULV8

• Accession Number:

  AAH28915.1

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-His-GST

• Sequence:

  Met 1-Ala474

• Field of Research:

  Epigenetics and Nuclear Signaling, Cancer

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  80.2 kDa

• Observed Molecular Weight:

  66 kDa

• Formulation:

  Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 8.0
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human