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CERTIFICATE OF ANALYSIS
#100 Cholera Toxin
(Azide-Free)
from Vibrio cholerae Inaba 569B

Price: 245 Euro

Contents:

Each vial of cholera toxin, when reconstituted to 1.0 ml with sterile distilled water, contains 1.0 mg of protein in: 0.05 M Tris, 0.2 M NaCl, 0.001 M Na₂EDTA at pH 7.5.
Solubility of cholera toxin, in water, decreases with time, following lyophilization.

Concentration:

Each vial contains 1 mg determined by extinction coefficient of 1.14 at 280 nm and 1 cm for 1 mg/ml.¹

Purity:

The preparation migrates as a single major band in disc gel electrophoresis (nondenaturing conditions). The R_280/260 of each preparation is reported.

Activity:

When examined in an ADP-ribosylation assay,^2,3 the specific enzymatic activity of each lot is at least as high as that of a standard cholera toxin preparation.

Packaging/Storage:

Lyophilized powder stoppered under vacuum. It is recommended that this material be stored at 4°C prior to and following reconstitution. DO NOT FREEZE.

Handling:

Good laboratory technique should be employed in the safe handling of this product. This requires observing the following practices:

1. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
2. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
3. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
4. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. List Biological Laboratories, Inc. is not liable for any damages resulting from the misuse or handling of this product.

FOR RESEARCH PURPOSES ONLY. NOT FOR HUMAN USE.

References:

1. Spangler, Brenda D. (1992) Microbiological Reviews 56 (4), 622-647.
2. Tsai, S.-C., Noda, M., Adamik, R., Moss, J., and Vaughan, M. (1987) Proc. Natl. Acad. Sci. USA 84, 5139-5142.
3. Tsai, S.-C, Noda, M., Adamik, R., Chang, P.P., Chen, H.-C., Moss, J., and Vaughan, M. (1988) J. Biol. Chem. 263, 1768-1772.

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