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Nitrofuran (AOZ) ELISA Kit

Brief
Nitrofuran (AOZ) ELISA Test Kit

Principle
This test kit is based on the competitive enzyme immunoassay for the detection of AOZ (3-anmino-2-oxazolidinone) in the tissue(chicken, fish,shrimp). The coupling antigen is pre-coated on the micro-well stripes. The AOZ in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against AOZ. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of AOZ in it. This value is compared to the standard curve and the content of the corresponding  AOZ is subsequently obtained.

Specifications
Sensitivity : 0.05ppb
Detection limit:
  Tissue and honey 0.1ppb
  Shrimp, fish(some interference in shrimp and fish) 0.2ppb
Recovery rate
       Shrimp and fish 90±10%
       Honey, chicken, meat/liver 75±15%
Cross-reaction rate:
  AOZ 100%
        AHD ﹤0.1%
        AMOZ ﹤0.1%
        SEM ﹤0.1%

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Last modified: 05/19/16