F-box

Item		Rabbit Anti-Emi1 Polyclonal Antibody

Antigen		Emi1


Catalog Number		24243

Quantity		100 ul

Complementary infromation

Human protein: Q9UKT4 - F-box only protein 5 (Early mitotic inhibitor 1)

Length: 447 aa, molecular weight: 50146 Da, CRC64 checksum: 196FBC2578F92120

     MSRRPCSCAL RPPRCSCSAS PSAVTAAGRP RPSDSCKEES STLSVKMKCD FNCNHVHSGL        60
     KLVKPDDIGR LVSYTPAYLE GSCKDCIKDY ERLSCIGSPI VSPRIVQLET ESKRLHNKEN       120
     QHVQQTLNST NEIEALETSR LYEDSGYSSF SLQSGLSEHE EGSLLEENFG DSLQSCLLQI       180
     QSPDQYPNKN LLPVLHFEKV VCSTLKKNAK RNPKVDREML KEIIARGNFR LQNIIGRKMG       240
     LECVDILSEL FRRGLRHVLA TILAQLSDMD LINVSKVSTT WKKILEDDKG AFQLYSKAIQ       300
     RVTENNNKFS PHASTREYVM FRTPLASVQK SAAQTSLKKD AQTKLSNQGD QKGSTYSRHN       360
     EFSEVAKTLK KNESLKACIR CNSPAKYDCY LQRATCKREG CGFDYCTKCL CNYHTTKDCS       420
     DGKLLKASCK IGPLPGTKKS KKNLRRL                                           447
//

General information

Entry name

FBX5 HUMAN

Accession number

Q9UKT4, Q8WV29, Q9UGC8

Created

Rel. 41, 28-FEB-2003

Sequence update

Rel. 41, 28-FEB-2003

Annotation update

Rel. 42, 10-OCT-2003

Description and origin of the Protein

Description

F-box only protein 5 (Early mitotic inhibitor 1).

Gene name(s)

FBXO5 OR FBX5 OR EMI1.

Organism source

Homo sapiens (Human).

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.

NCBI TaxID

9606

References

[1]

Cenciarelli,C., Chiaur,D.S., Guardavaccaro,D., Parks,W., Vidal,M., Pagano,M.,
Identification of a family of human F-box proteins.
(1999) Curr. Biol. 9:1177-1179

Position

SEQUENCE FROM N.A.

Medline

20003060

PubMed

10531035

[2]

Hsu,J.Y., Reimann,J.D.R., Sorensen,C.S., Lukas,J., Jackson,P.K.,
The E2F-dependent accumulation of EMI1 regulates S phase entry by inhibiting APCCdh1.Submitted FEB-2002 to the EMBL GenBank DDBJ databases

Position

SEQUENCE FROM N.A.

[3]

Phillimore,B., Submitted DEC-1999 to the EMBL GenBank DDBJ databases

Position

SEQUENCE FROM N.A.

[4]

Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S.N., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J.M., Marra,M.A.,
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
(2002) Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903

Position

SEQUENCE FROM N.A.

Comments

TISSUE=Placenta;

Medline

22388257

PubMed

12477932

Comments

FUNCTION

Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.

SUBUNIT

Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity).

SIMILARITY

Contains 1 F-box domain.

Database cross-references

EMBL

AF129535; AAF04469.1; -.

AY079515; AAL86610.1; -.

AL080276; CAB65993.1; -.

BC018905; AAH18905.1; -.

Genew

HGNC:13584; FBXO5.

MIM

606013; -.

GO:0004840; F:ubiquitin conjugating enzyme activity; TAS.

GO:0004842; F:ubiquitin-protein ligase activity; TAS.

GO:0006508; P:proteolysis and peptidolysis; TAS.

InterPro

IPR001810; F-box.

Pfam

PF00646; F-box; 1.

PROSITE

PS50181; FBOX; FALSE_NEG.

Keywords

Ubl conjugation pathway;

Features

Key	Begin	End	Length	Description
DOMAIN	250	296	47	F-box.
CONFLICT	107	107	1	Q -> E (in Ref. 4).
CONFLICT	304	329	26	Missing (in Ref. 3).

HOMOLOGENE ENTRY

H.sapiens -FBXO5

F-box only protein 5
UniGene | LocusLink | MIM | MapViewer | NM_012177.2

POSSIBLE HOMOLOGOUS GENES

M.musculus -Fbxo5

F-box only protein 5
UniGene | LocusLink | MGI | MapViewer | NM_025995.1

R.norvegicus -LOC292263

similar to Fbxo5 protein
UniGene | LocusLink | MapViewer | XM_214756.2

S.scrofa -Ssc.24858

Transcribed sequence with moderate similarity to protein refNP_036309.1 (H.sapiens) F-box only protein 5; F-box protein Fbx5; homolog of xenopus EMI1 [Homo sapiens]
UniGene | CK466339.1 | BG733555.1 | CK468447.1

B.taurus -Bt.986

Transcribed sequences
UniGene | CB435835.1 | CB435564.1

GeneReport for:
AF129535

FBXO5

F-box only protein 5
UniGene, LocusLink, OMIM, GenAtlas, GeneCard, Ensembl, MapView, Genome Browser

Aliases

EMI1, XENOPUS, HOMOLOG OF
F-box protein Fbx5

Chromosomal Location

Chromosome/Cytoband

6q25-q26

LocusLink Information

Locus Link Summary

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex.

Annotations

Gene Ontologies

Ontology	Annotation	Evidence	Source
Molecular Function	Ubiquitin conjugating enzyme activity	TAS	GOA
Molecular Function	Ubiquitin-protein ligase activity	TAS	GOA
Biological Process	Proteolysis and peptidolysis	TAS	GOA

UniGene & EST Expression Information

UniGene Cluster

Hs.272027 from Build No. 166 , Released on 2004-01-17

Normalized expression distribution for tissue type

Top ten [of 38]

[Help]

Tissue	Normalized Expression (%)	Cluster Clones : Tissue clones
juvenile granulosa tumor:	8.69	1:2247
malignant melanoma, metastatic to lymph node:	7.26	1:2691
metastatic prostate bone lesion:	6.77	1:2884
lung carcinoma:	5.71	1:3424
germinal center B cell:	4.73	9:37172
kidney tumor:	4.01	1:4869
squamous cell carcinoma:	3.78	2:10343
sciatic nerve:	3.71	1:5269
ovarian tumor:	3.71	2:10544
from acute myelogenous leukemia:	3.56	1:5485

SAGE (NCBI)

Go to Gene-to-tag Mapping at NCBI

Upstream Genomic Sequence

TRASER

Upstream genomic sequence for F-box only protein 5

Representative mRNA Sequences

UniGene

NM_012177

LocusLink RefSeq

Accession	Description
NM_012177	NA

Alias PubMed Search

PubMed

name	begin	end	E-value
low complexity	11	23	-
low complexity	151	167	-
Pfam:F-box	245	296	1.20e-02

ACTIVATE: SOSUI analysis
This amino acid sequence is of a SOLUBLE PROTEIN.

 	 73.9 %: mitochondrial
	 17.4 %: nuclear
	  4.3 %: cytoskeletal
	  4.3 %: cytoplasmic

Activate MapView for: Q9UKT4

Human protein: Q9UKT4 - F-box only protein 5 (Early mitotic inhibitor 1)

Send mail to lieven@gentaur.com with questions or comments about this web site. Copyright © 2008 Gentaur Molecular Products Page modified by Acid Dragon (AC) Last modified: 08/27/08

Send mail to lieven@gentaur.com with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Page modified by Acid Dragon (AC)
Last modified: 08/27/08