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Technical

The advantages of using Enhanced Autoradiography with EA-Wax have been evaluated in many real-world experimental situations. Full descriptions and autoradiographic images are available for viewing from this page. For your convenience, these experiments are also described in full in the EA Wax Information Pack. You can request copies of this from GenTaur or download an electronic version from this web site.

 

Click on the text link to view details of each of the experiments listed. Clicking on the images link will load a  large size image of the autoradiograms into a separate browser window. When you are finished viewing the image just close the browser window.

 

Experimental Evaluations

  • Separation of Tritiated(3H) and Carbon-14(14C) Monosaccharides

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  • 3H-DNA Dot Blo

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  • 3H-Methyltransferase Assa

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  • 3H-labelled PCR product

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  • Detection of 3H on TLC plate

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  • Western blot analysis of 14C-labelled membrane protei

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  • 2D gel analysis of 35S-methionine labelled proteins in S. typhimurium SL134

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  • 2D gel analysis of 35S-methionine labelled proteins in Campylobacter jejuni

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  • 2D gel analysis of E.coli global protein change during GroELS depletion

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Summary of the Benefits of Enhanced Autoradiography.

1.        Tritium(3H) has always been the isotope of choice for both research, and routine laboratory use, but it has always been the most difficult to detect. Enhanced Autoradiography solves the detection problem. A revolution is now possible which will effect many branches of science.

2.        Enhanced Autoradiography substantially reduces the time interval between an experiment or procedure being performed and the results being obtained.

3.        The process is in general non-destructive of the sample, so further analysis can be performed on the same sample.

4.        The invention allows the accurate quantitation of radioactivity particularly that of tritium(3H) which currently is not possible.

5.        High cost equipment, with high cost consumables and maintenance are not required. Equipment found in most laboratories, an oven and refrigerator, are all that are required in addition to the EA-Wax. This of course means that Enhanced Autoradiography will open doors, to research and routine science, in the Third World countries as well as developed countries.

6.        The materials used in Enhanced Autoradiography are low cost and environmentally friendly. They are also inherently safe to handle, store, transport and dispose of.

7.        The final method of detection is X-ray film, which gives the highest possible resolution.

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