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• FPC or Phycocyanin is a phycobiliprotein isolated from Spirulina sp., a blue-green algae. Like other phycobiliproteins, C-Phycocyanin is fluorescent, with an extremely high absorbtivity, a high quantum efficiency, a large Stokes shift and excitation and emission bands at visible wavelengths. It is a stable protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics.

 

• PE or Phycoerythrin is a phycobiliprotein isolated from red algae developed as a source of choice because it yields one of the most highly fluorescent. Like other phycobiliproteins, R-PE is fluorescent, with an extremely high absorbtivity, a high quantum efficiency, a large Stokes shift and excitation and emission bands at visible wavelengths. It is a stable protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics.

 

To be used in fluorescence analysis, FPC or PE has the unique advantages compared with traditional chemic fluorescent dye, they main lie below:

1) It possesses wider absorption spectrum when it is in the wider range of PH, and it is easier to select appropriate excitation wavelength in order to take fluorescence emission with efficiency, in addition, it has differential fluorescence-emission peak;

2) Absorbance and fluorescence quantum productivity is very high, the fluorescence is strong and stable, the sensitivity is high, too;

3) It has smaller fluorescence background and the fluorescence emission is not easy to quench, its fluorescence retention period is longer;

4) Its water solubility is excellent, and it’s prone to cross link other molecule(s), and the nonspecific adsorption is small;

5) It is purified from pure natural halobios and no toxicity and side effect, no radioactivity. And the operation is very safe.

 

Because of their properties, phycobiliproteins have been used in a variety of immunological assays and as fluorescent labels for cell-sorting. In addition, because of the high molar absorbtivity of these proteins at visible wavelengths, they are convenient markers in such applications as gel electrophoresis, isoelectric focusing and gel exclusion chromatography.

 

Ordering Information:

FPC        PE

1mg     120 Euro     130 Euro

5mg     470 Euro    490 Euro

25mg   2000 Euro   2150 Eur

 

AW-PC006    Fluorescence Phyococyanin(FPC)   

                  1mg     5mg        25mg

From Spirulina sp.,                                              

Blue lyophilized powder

A620 / A280 > 4.5

 

Highly purified,lyophilized powder containing sucrose, dithioerythritol and sodium azide as preservatives

            Protein approx. 30% by Lowry

 

            Ref.: Boussiba, S. and A. E. Richmond. C-Phycocyanins as a storage protein in the blue-green alga Spirulina platensis. Arch. Microbiol. 125:143 147 (1980).

Glazer, A. N. Phycobilisomes: structures and dynamics. Ann. Rev. Microbiol. 36:173 198 (1982).

Kronick, M. N. The use of phycobiliproteins as fluorescent labels in immunoassay. J. Imm. Meth. 92:1 13 (1986).

 

 

AW-PE002    Phycoerythrin(PE)     

          1mg           5mg              25mg

From Spirulina sp.,                                              

Red lyophilized powder

A620 / A280 > 5.0

 

Highly purified,lyophilized powder containing sucrose, dithioerythritol and sodium azide as preservatives

            Protein approx. 30% by Lowry

 

            Ref.: Boussiba, S. and A. E. Richmond. C-Phycocyanins as a storage protein in the blue-green alga Spirulina platensis. Arch. Microbiol. 125:143 147 (1980).

Glazer, A. N. Phycobilisomes: structures and dynamics. Ann. Rev. Microbiol. 36:173 198 (1982).

Kronick, M. N. The use of phycobiliproteins as fluorescent labels in immunoassay. J. Imm. Meth. 92:1 13 (1986).

 

 

AW-APC007    Allophycocyanin(APC)     

          1mg        5mg           25mg

From Spirulina sp.,                                              

Blue lyophilized powder

A650 / A280 > 4.0

 

Highly purified,lyophilized powder containing sucrose, dithioerythritol and sodium azide as preservatives

            Protein approx. 30% by Lowry

 

           Ref.:1.Chou, H.N. and J.M. Jeng, 1993. High performance liquid chromatography of biliprotein from Bangia atropurpurea 'Conchocelis'. Hydrobiologia 260/261:571-576. 

2.Glazer, A. N. 1994, Phycobiliproteins - a family of valuable, widely used fluorophores, J. Appl. Phycol. 6: 105-112.

3.Kronick, M. N. 1986, The use of phycobiliproteins as fluorescent labels in immunoassay, J. Imm. Meth. 92: 1-3.