Osteoclasts

Culture plate for measuring the resorption ability of osteoclasts

Ca-P thin film-coated surface

arrow head: resorption pit /arrow: osteoclast

> Description

	Osteoclast is a large multinucleate cell formed from differentiated macrophage, responsible for breakdown of bone. It could be characterized by the multi-nucleation, TRAP positive and formation of resorption pit.
	OAAS^TM is a culture plate to measure the activity of the osteoclast in bone research. Due to its bottom coated with low crystalline of carbonated calcium phosphate, OAAS^TM resembles human bone and will be a perfect substitute for dentin slice ever.

> Advantage of OAAS^TM


Activities are observed with bare eyes during its cultivation

	You will have reliable data because calcium phosphate is coated as the substrate for osteoclast, and it is more comparable to human bone than the other product coated with synthetic hydroxyapatite.
	While you culture the osteoclast precursor cell, you may observe the differentiation. (Culture period: 2-3days for matured osteoclast, 7-12 days for precursor cell)
	After removing the matured osteoclasts by NaOCl (sodium hypochlorite), you may measure the resorption pit margin by an image analyzer.
	Due to clear calcium carbonate coated, you may observe the resorption area under light microscopy without SEM and even with your bare eyes during culture.
	OAAS^TM can be used for TRAP staining of osteoclasts in situ.


	Figure 1, 2: Resorption areas in a culture dish after removing osteoclasts
	Figure 3: TRAP staining of osteoclasts in situ and resorption area
	Figure 4: SEM picture of a osteoclast and resorption area

> Specification

Category	Description
Product type	Culture Plate
Growth surface	Useful for tissue culture
Coating type	Low crystalline of carbonated calcium phosphate
Lid	Yes
Shape	Flat-bottom
Size	4well, 24well, 48well
Volume	OAAS-4	4well	2ml
	OAAS-24	24well	2ml
	OAAS-48	48well	1ml
Growth Area	OAAS-4	4well	1.9cm²
	OAAS-24	24well	1.9cm²
	OAAS-48	48well	1cm²
Material	White with clear bottom
Trademark	OAAS
Special Order	Special order such as trace elements incorporated, different crystallinity, variable thickness, specific protein bound, 96-well plate, or chamber slide / cover slips, may be accepted. Please contact us for further information.

How to use OAAS

Image Analysis for OAAS

Papers where OAAS is used