Mouse cytokine Array II

Mouse Cytokine Microarray II (Fluorescent)

Mouse cytokine microarray is a protein array kit

designed to screen diverse mouse samples, such as cell

lysates, serum, plasma and tissue culture supernatants,

for the presence of 14 different cytokines

simultaneously. Each slide in the kit contains eight

identical sub-arrays of 14 capture antibodies in

quadruplicate and supports the analysis of up to eight

40-µL samples. A cocktail of biotinylated detection

antibodies is provided. The antibody pairs bind the

cytokine antigen in a sandwich format. Cytokine

detection is achieved with the addition of streptavidinfluorophore

conjugate. Positive and negative controls

spotted within each sub-array allow for assay

validation. In addition, two-color detection is allowed

when samples are labeled with fluorophores.

Features and Benefits

• Eliminate blocking step and ready for use

• Screen 14 cytokines simultaneously in one mouse

sample

• Process up to eight samples on one slide

• Accept low sample volume (40 µL) per sub-array

• Compatible with diverse sample types

• Allow two-color detection (Cy3 & Cy5)

• Comparable with ELISA’s sensitivity, specificity

and dynamic range

• Fully customizable arrays upon experimental

needs

Mouse Cytokines Detected

IL-1â

IL-2

IL-4

IL-5

IL-6

IL-10

IL-13

GM-CSF

IFN-γ

RANTES

MCP-1

TNF-α

VEGF

IL-12 (p70)

Microarray Image

Mixed recombinant proteins (IL-1β, IL-4, IL-6 and GM-CSF)

were applied to each sub-array at different concentrations.

PC-positive control; NC-negative control.

Assay Performance of Mouse Cytokine Microarray

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Send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Site powered by Acid Dragon (AC)
Last modified: 07/15/09