Description: G418 is an aminoglycoside used as a selective agent of transfected mammalian, yeast, plant and bacteria cells. Resistance is conferred by the bacterial gene for aminoglycoside-3'-phophotransferase that can be expressed in eukaryotic cells. G418 is related to Gentamycin.
Chemical Formula: C20H40O10N4.2H2SO4
Molecular Weight: M.W. 692.7
CAS Number: CAS [108321-42-2] RTECS CB9378500
Active Product: Y
Appearance: Liquid
Purity: Potency: >700 ug/mg
Handling: Store in Tightly Sealed Vial. Protect from Light.
Storage: +4°C.
Shipping: Express Courier
Bulk Quantity: Available, Please Inquire
 

White solid. Aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neo^r/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418. Purity: ≥98% by TLC. Potency:≥730 mg/mg. pH 4.0 -6.0. RTECS CB9378500, CAS 108321-42-2.

Ref.: Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189; Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245; Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY; Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.

IDENTIFICATION OF THESUBSTANCE/PREPARATIONAND COMPANYPRODUCT NAME:Antibiotic G-418

PRODUCT CODE:11379

EEC NUMBER:None

SUPPLIER:Emergency Contact:GENTAUR

COMPOSITION/HAZARDOUS COMPONENTSHAZARDCAS NO.

%WTTLVCHIP R & S PhrasesAntibiotic G-418108321-42-2100%---R:36/37/38 Irritating to eyes,respiratory system and skinR:42/43 May causesensitization by inhalation andskin contact.S:22 Do not breath dustS:36/37 Wear suitableprotective clothing and glovesHAZARDS IDENTIFICATIONCHIPIrritant HCSIrritant

FIRST-AID MEASURESEYES: Flush with water for 15 minutes. Seek medical advice if irritation persists.

SKIN: Flush with water, then wash thoroughly with soap and water. Remove conta minated clothing and wash before reuse. Seek medical attention if irritation persists.INHALATION: Remove the victim from exposure and move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. Keep victim quiet and warm. Seek immediate medical attention.

INGESTION: Drink water and seek immediate medical attention. Avoid alcoholic beverages. Never give anything by mouth to an unconscious person.

FIRE-FIGHTING INFORMATIONUse media suitable to extinguish the supporting or surrounding fire. Wear NIOSH (or equivalent) approved selfcontained breathing apparatus. For small fires only: use carbon dioxide, dry powder or foam. No unusual fire orexplosion hazards known.Flash Point = No data Available.

ACCIDENTAL RELEASE MEASURESWear appropriate personal protective equipment and clothing including lab coat , safety glasses, gloves andNIOSH-approved dust mask. Collect in a manner that does not create dust and place in a suitable wastecontainer. Avoid contact of material with skin or eyes. Use adequat e ventilation.

HANDLING AND STORAGEWear appropriate personal protective equipment and clothing including lab coat , safety glasses, gloves andNIOSH-approved dust mask. Avoid contact of material with skin or eyes. Use adequate ventilation. Store at+4°C.PERSONAL PROTECTIONWear appropriate personal protective equipment and clothing including lab coat , safety glasses, gloves andNIOSH-approved dust mask. A qualified industrial hygienist should evaluate the need for respiratory protection.Use respiratory protection approved by NIOSH (or equivalent) and appropriate to the haza rd. Avoid contact ofmaterial with skin or eyes. Mechanical ventilation or local exhaust as needed to control exposure to dust,vapors or mists. Access to a safety shower and eye-wash.

PHYSICAL AND CHEMICAL PROPERTIES

Appearance: White to off-white odorless powder Boiling Point: No data availableVapor Pressure: No data available Vapor Density: No data availableSolubility (Water): Slightly soluble Specific Gravity: 1g/cm3Percent Volatile: No data available Evaporation Rate: No data availableChemical formula: C20-H40-N4-O10 øP2H2SO4STABILITY AND REACTIVITYProduct is stable. Hazardous polymerization will not occur.

TOXICOLOGICAL INFORMATIONEFFECTS OF OVEREXPOSURE:EYES: Contact may cause irritation.SKIN: Contact may cause irritation, dermatitis and hypersensitive individuals may experience an allergic reaction.

INHALATION: May cause irritation, and hypersensitive individua ls may experience an allergic reaction.INGESTION: Chronic ingestion or excessive dosage may cause allergic rea ction, skin rash and/or labored breathing.

TARGET ORGAN(S): Lungs.ADDITIONAL INFORMATION:RTECS: No data available.Definitions: RTECS = Registry of Toxic Effects of Chemic al Substances.

ECOLOGICAL INFORMATIONNo information availableDISPOSAL CONSIDERATIONSDispose of material in accordance with applicable local, stat e, and federal regulations.

TRANSPORTATION INFORMATIONUS DOT / IATA: No applicable information.

REGULATORY INFORMATIONRCRA - No applicable information.SARA 302 - No applicable information.SARA 313 - No applicable information.EPA TSCA Section 8(b) - No applicable information.Exposure Limits - Not established.California Proposition 65 - No applicable information.