Protein Kinase

Protein Kinases
Unless indicated otherwise, all products below are supplied per vial in 50 ml 50 mM Tris-HCl, pH 7.0 buffer containing 14 mM b-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, 50% glycerol, except that, for AK, 65% ethyleneglycol is included instead of glycerol, and the buffer also contains 0.1% Brij-35. Maintain all preparations at -70°C. Avoid repeated thawing. One unit of protein kinase is the amount of the enzyme that incorporates 1 nmol of phosphoryl groups into the indicated substrate per min. For ordering information, click here.

AK; Auto-Activated Protein Kinase
GLO111 (10 ml, 50 ng, $285)

Scarce enzyme which undergoes rapid autophosphorylation and dramatic (15-30-fold) activation. The enzyme phosphorylates and inactivates PP2A (GLO130-132, protein phosphatases). AK is involved in cytoskeleton organization and other signal transduction processes. The purified preparations appear related to the catalytic domain of p21-activated p65^PAK protein kinase (GLO116, this page), and is produced in cells undergoing apoptosis via a caspase-dependent pathway. Source bovine kidney. Purity > 90% by SDS-PAGE. Apparent Mr ~ 36-kDa. Specific activity ~ 17,000 units/mg with MBP as substrate. For MBP, see GLO126, kinase substrates).

ref: 1. Proc Natl Acad Sci USA 90: 2500 2. J Biol Chem 268: 11193 3. J Cell Physiol 178: 397 4. J Biol Chem 276: 14909

AK assay kit ^NEW!
GLO111-01 ($385)

Useful for determining the activity of AK or related protein serine kinase. Can be used to evaluate the effect of a test substance on AK activity, or to identify new physiological substrates of the enzyme. Kit provides for 50 assays. Assay is based on the reaction of AK with MBP (GLO126, kinase substrates) and g-³²P-ATP. Kit includes directional insert, and contains solutions of MgATP, MBP, assay buffer, and purified AK sample. Store solutions at -70°C. Avoid repeated thawing. g-³²P-ATP and trichloroacetic acid are not provided with kit.

ref: 1. Proc Natl Acad Sci USA 90: 2500 2. J Biol Chem 268: 11193
3. J Cell Physiol 178: 397 4. J Biol Chem 276: 14909

CK-2; Casein Kinase-2
GLO112 (1 mg, $285)

A tetrameric protein serine/threonine kinase that is ubiquitously distributed in eukaryotes. CK-2 is an important component of signaling pathways, controls the growth and division of cells, and regulates the activity of a variety of regulatory nuclear proteins including oncoproteins and transcription factors, as well as enzymes involved in metabolism. Source bovine kidney. Purity > 90% by SDS-PAGE. Subunits ~ 39- and 26-kDa. Specific activity ~ 2400 units/mg with casein as substrate.

ref: 1. Biochim Biophys Acta 1054: 2672 2. Biochem J 232: 767 3. Biochim Biophys Acta 1054: 267 3. Biochem Biophys Res Commun 166: 449 4. Arch Biochem Biophys 262: 574

CK-2 assay kit ^NEW!
GLO112-01 ($385)

Useful for determining the activity of CK-2 or related protein serine kinase, to evaluate the effect of a test substance on CK-2 activity, or to identify new physiological substrates of the enzyme. Kit provides for 50 assays. Assay is based on the reaction of CK-2 with casein and g-³²P-ATP. Kit includes directional insert, and contains solutions of MgATP, casein, assay buffer, and purified CK-2 sample. Store solutions at -70°C. Avoid repeated thawing. g-³²P-ATP and trichloroacetic acid are not provided with kit.

ref: ref: 1. Biochim Biophys Acta 1054: 2672 2. Biochem J 232: 767 3. Biochim Biophys Acta 1054: 267 3. Biochem Biophys Res Commun 166: 449 4. Arch Biochem Biophys 262: 574

MBPK-1; Myelin Basic Protein Kinase-1
GLO113 (100 ng, $285)

Distinct MAPK family member. MAPK is involved in regulating numerous cellular processes including cell growth and differentiation. In contrast to other MAPK’s, MBPK-1 is not inactivated by protein tyrosine phosphatases. MBPK-1 and MBPK-2 phosphorylate and activate cPK (GLO117, this page). Source bovine kidney. Purity > 90% by SDS-PAGE. Apparent Mr ~ 40-kDa. Specific activity ~ 300 units/mg with MBP as substrate. For MBP, GLO126, kinase substrates.

ref: J Biol Chem 268: 15298

MBPK-2; Myelin Basic Protein Kinase-2
GLO114 (100 ng, $285)

Distinct MAPK family member. MAPK is involved in regulating numerous cellular processes including cell growth and differentiation. In contrast to other MAPK’s, MBPK-2 is not inactivated by protein tyrosine phosphatases. MBPK-1 and MBPK-2 phosphorylate and activate cPK (GLO117, this page). Purity > 90% by SDS-PAGE. Apparent Mr ~ 40-kDa. Specific activity ~ 1100 units/mg with MBP as substrate. For MBP, see GLO126, kinase substrates.

ref: J Biol Chem 268: 15298

MBPK assay kit ^NEW!
GLO113-01 ($385)

Useful for determining the activity of MBPK-1, MBPK-2 or related protein serine/threonine kinase. Can be used to evaluate the effect of a test substance on MBPK activity or to identify new physiological substrates of MBPK-1. Kit provides for 50 assays. Assay is based on the reaction of kinase sample with MBP (GLO126, kinase substrates) and g-³²P-ATP. Kit includes directional insert, and contains solutions of MgATP, MBP, assay buffer, and purified MBPK-1 sample. Store solutions at -70°C. Avoid repeated thawing. g-³²-ATP and trichloroacetic acid are not provided with kit.

ref: J Biol Chem 268: 15298

c-raf-1
GLO115 (100 ng, $285)

c-raf-1 is the first upstream kinase in the MAPK signal transduction pathway. This pathway is involved in regulating numerous cellular processes including cell growth and differentiation. Source recombinant human. Purity > 10% by SDS-PAGE. Specific activity 20 units/mg with MEK as substrate.

ref: 1. Methods Enzymol. 255: 279 2. Science 264: 1463

p65^PAK; p21-Activated Protein Kinase
GLO116 (100 ng, $285)

The p21-activated p65^PAK protein kinase is involved in cytoskeleton organization, apoptosis and signal transduction processes. The enzyme has been found associated with PP2A (GLO130-132, protein phosphatases) and ribosomal protein S6 kinase. The catalytic domain of the enzyme is produced in apoptotic cells via a caspase-dependent pathway and does not require rac and cdc42 (GLO128, modulator proteins) for activity. Source recombinant human. Purity ~ 10% by SDS-PAGE. Specific activity ~ 200 units/mg with MBP as substrate. For MBP, see GLO126, kinase substrates.

ref: 1. Nature 367: 40 2. J Biol Chem 270: 21695 3. J Cell Physiol 178: 397 4. J Biol Chem 276: 14909

cPK; Cytosolic Protamine Kinase
GLO117 (100 ng, $285)

Distinct insulin-stimulated cytosolic protein kinase which phosphorylates eIF-4E (GLO118, kinase substrates) at physiological sites. Regulates protein synthesis and numerous other cellular processes. Source bovine kidney. Purity > 90% by SDS-PAGE. Apparent Mr ~ 45-kDa. Specific activity ~ 17,000 units/mg with protamine sulfate as substrate.

ref: 1. J Biol Chem 264: 6412 2. J Biol Chem 268: 15298 3. Biochem J 287: 1019

cPK assay kit ^NEW!
GLO117-01 ($385)

Useful for determining the activity of cPK or related protein serine/threonine kinase. Can be used to evaluate the effect of a test substance on cPK activity or to identify potential new physiological substrates of the enzyme. Kit provides for 50 assays. Assay is based on the reaction of cPK with protamine sulfate and g-³²P-ATP. Kit includes directional insert, and contains solutions of MgATP, protamine sulfate, assay buffer, and purified cPK sample. Store solutions at -70°C. Avoid repeated thawing. g-³²P-ATP and trichloroacetic acid are not provided with kit.

ref: 1. J Biol Chem 264: 6412 2. J Biol Chem 268: 15298 3. Biochem J 287: 1019

BCKDH Kinase Kit ^NEW!
GLO125-01 ($385)

Effective for determining the activity of BCKDH Kinase and to evaluate the effects of test substances on its activity. Kit provides for 50 assays. Assay is based on the reaction of the kinase with BCKDH complex and ATP. Kit includes directional insert, and contains solutions of Mg , ATP, assay buffer, and purified sample of BCKDH complex. Store solutions at -70°C. Avoid repeated thawing. g-³²P-ATP and trichloroacetic acid are not provided with kit. For BCKDH complex, see GLO125, kinase substrates).

ref: 1. Methods Enzymol 166: 321 2. Proc Natl Acad Sci USA 81: 4335 3. J Biol Chem 262: 5129