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Product Code
Product Name
Unit Price

0100

Media Optimization Kit™

€ 67,7

0100-APF

APF Media Optimization Kit™

€ 67,7

0128

Media Optimization Kit™, Liquid

€ 86,6

0129

APF Media Optimization Kit™, Liquid

€ 86,6

0101

LB (Luria)

€ 63,0

0102

LB (Lennox)

€ 59,6

0103

LB (Miller)

€ 56,5

0104

Turbo Broth™

€ 60,6

0104-S

Turbo Broth™, Single Packs - box of 10

€ 60,6

0105

Superior Broth™

€ 60,6

0105-S

Superior Broth™, Single Packs - box of 10

€ 60,6

0106

Power Broth™

€ 61,9

0106-S

Power Broth™, Single Packs - box of 10

€ 61,9

0107

Hyper Broth™

€ 58,0

0107-S

Hyper Broth™, Single Packs - box of 10

€ 58,0

0108

Glucose M9Y

€ 50,8

0108-S

Glucose M9Y, Single Packs - box of 10

€ 50,8

0109

Glucose Nutrient Mix

€ 38,5

0110

Turbo Prime Broth™

€ 63,8

0110-S

Turbo Prime Broth™, Single Packs - box of 10

€ 63,8

0111

Superior Prime Broth™

€ 63,8

0111-S

Superior Prime Broth™, Single Packs - box of 10

€ 63,8

0112

Power Prime Broth™

€ 63,8



Enzyme Assays

 1-4 kits

0201

PDQ Protease Assay

 €    130.00

 

Media Optimization Kit™

Cat. No. 0100

Intended Use

The Media Optimization Kit™ is used for determining the best medium formulation for the expression of recombinant .  Our experience in working with a wide variety of recombinant proteins has shown that the type of medium used to produce a given protein can have a significant impact on the expression level.  The Media Optimization Kit™ allows the researcher to determine which medium formulation is best suited to his/her application.  Each kit contains enough media to prepare 1 liter of each of the six media used by the scientific staff at AthenaES to optimize the expression of recombinant proteins.

Kit Components"Verdana"> Kit Components

      Powder Mixes for 1 liter of:

  • Glucose M9Y

  • Turbo Broth™

  • LB Broth, Miller

  • Power Broth™

  • Hyper Broth™

  • Superior Broth™

Unit Size

Cat. No.

1 kit

2 kits

6 kits

0100-1

0100-2

0100-6

 

APF Media Optimization Kit™ Cat. No. 0100-APF

Intended Use

The Animal Product Free Media Optimization Kit™ is used for determining the best medium formulation for the expression of recombinant proteins in E. coli.  This kit is based on our original Media Optimization Kit™ except the media are certified "animal product free."  Only LB Broth, which serves as a reference medium, is made with an animal-derived raw material.  All the other media are APF Certified.

Kit Components"Verdana"> Kit Components

      Powder Mixes for 1 liter of:

  • Glucose M9Y

  • Turbo Prime Broth™

  • LB Broth, Miller

  • Power Prime Broth™

  • Hyper Broth™

  • Superior Prime Broth™

Unit Size

Cat. No.

1 kit

2 kits

6 kits

0100-APF-1

0100-APF-2

0100-APF-6

 

LB Broth - Luria, Lennox, and Miller

Cat. Nos. 0101, 0102, 0103

LB Broth media formulations have been an industry standard for the cultivation of Escherichia coli since the 1950’s[1],[2],[3].  These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins[4],[5].  The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements.  The three formulations differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions.  The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin™. 

Formulas – grams per liter

Ingredient

Luria

Lennox

Miller

Tryptone

10

10

10

Yeast Extract

5

5

5

NaCl

0.5

5

10

Solid medium contains 15 g bacteriological agar per liter.

Method of Preparation

1.        Dissolve 15.5, 20 and 25 grams, LB-Luria, LB-Lennox, LB-Miller, respectively, in 1 liter of deionized water.  For solid medium, add 15 g bacteriological agar.

2.        Autoclave at 121°C for 15 min.  Store liquid medium at 4°C.

3.        For solid medium, allow to cool to 45°C.  Supplement with antibiotic as needed.  Pour about 30 ml per 100 mm petri dish.  Allow to cure overnight.  Store at 4°C in plastic bags.

 Note:  To promote faster growth, the medium can be supplemented with 10 ml 10% sterile glucose or 40 ml 10% sterile glycerol.

Media

Unit Size

Cat. No.

 

LB-Luria

500 g

2 pack

6 packa

0101-1

0101-2

0101-6

 

LB-Lennox

500 g

2 pack

6 packa

0102-1

0102-2

0102-6

 

LB-Miller

500 g

2 pack

6 packa

0103-1

0103-2

0103-6

 

 a Six pack sold as six 500 g containers to ensure freshness during storage, prevent unintentional contamination during use and prevent caking of powder.

 

Turbo Broth

Cat. No. 0104

Turbo Broth™ is used for the cultivation of recombinant strains of E. coli.  This medium has been developed to improve the yield of recombinant proteins.  The carbon source is glycerol which is not metabolized into acetic acid.  It has a rich nutrient base which provides amino acids, vitamins, inorganic and trace minerals at levels higher than those of LB Broth.  The medium is buffered at pH 7.2±0.2 with potassium phosphate to prevent a drop in pH and to provide a source of phosphate.  These modifications permit higher cell yields than can be achieved with LB.  For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth.  Cell yields are typically 4 to 5 times that of LB Broth in shake flask cultures depending on the characteristics of the strain.  Plasmid copy numbers are higher, which can also enhance protein expression.  This medium is suitable as the base for fermentation cultures.  Biomass yields will depend on the process employed and the strain.  A typical bench-scale fermentation operated in a batch mode can achieve an OD600 of 10 to 12 depending on the strain.

Formula – Proprietary

Method of Preparation

  1. Dissolve 47.6 grams in 1 liter deionized water.

  2. Add 4 ml glycerol.

  3. Dispense as required.

  4. Autoclave at 121°C for 15 min

Media

Unit Size

Cat. No.

Turbo Broth™

500 g

2 pack

6 packa

0104-1

0104-2

0104-6

 a Six pack sold as six 500 g containers to ensure freshness during storage, prevent unintentional contamination during use and prevent caking of powder.

 

Superior Broth™

Cat. No. 0105

Superior Broth™ is a proprietary medium formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli.  The medium is a complex, rich formulation which supplies a nitrogen source, vitamins, and moderate glucose levels.  The medium is buffered at pH 7.2±0.2 to prevent acidification of the medium during metabolism of the glucose.  For expression of recombinant proteins, induction should be done at cell densities twice that of LB Broth.  Cell yields are typically about 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain.  Plasmid copy numbers are the highest among the AthenaES family of media.  This medium is an excellent choice for large-scale plasmid preparations.  Biomass yields will depend on the process employed and the strain.

Formula – Proprietary

Method of Preparation

  1. Dissolve 35 grams in 1 liter deionized water.

  2. Dispense as required.

  3. Autoclave at 121°C for 15 min.

Media

Unit Size

Cat. No.

Superior Broth™

500 g

2 pack

6 packa

0105-1

0105-2

0105-6

 a Six pack sold as six 500 g containers to ensure freshness during storage, prevent unintentional contamination during use and prevent caking of powder.

 

Power Broth™

Cat. No. 0106

Power Broth™ is a proprietary medium formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli.  The medium is a complex, rich formulation which supplies amino acids, vitamins, and a carbon source at higher levels than Terrific Broth5.  The medium is buffered at pH 7.2±0.2.  For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth.  Cell yields are typically 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain.  The amount of soluble protein accumulated for otherwise insoluble proteins is increased by growing in Power Broth™.  This medium is suitable as the base for fermentation cultures.  Biomass yields will depend on the process and the strain employed.

Formula – Proprietary

Method of Preparation

  1. Dissolve 52 grams in 1 liter deionized water.

  2. Add 4 ml of glycerol.

  3. Dispense as required.

  4. Autoclave at 121°C for 15 min.

Media

Unit Size

Cat. No.

Power Broth™

500 g

2 pack

6 packa

0106-1

0106-2

0106-6

 a Six pack sold as six 500 g containers to ensure freshness during storage, prevent unintentional contamination during use, and prevent caking of powder.

 

Hyper Broth™

Cat. No. 0107

Hyper Broth™ is a proprietary medium formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli.  The medium is a complex, rich formulation which supplies amino acids, vitamins, and glucose at high levels.  This medium produces the highest level of biomass production.  The medium is buffered at pH 7.5±0.2.  This medium has the highest buffering capacity of the expression media set, which permits high glucose levels.  For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth.  Cell yields are typically 4 to 5 times that of LB Broth in shake flask cultures depending on the characteristics of the strain.  Plasmid copy numbers are higher than for LB, which can facilitate protein expression.  This medium is suitable as the base for fermentation cultures and is excellent for feedbatch applications.  Biomass yields will depend on the process and the strain employed.

Formula – Proprietary

Method of Preparation

  1. Dissolve 44.5 grams in 950 ml deionized water.

  2. Dispense as required.

  3. Autoclave at 121°C for 15 min.

  4. Aseptically add 50 ml of Glucose Nutrient Mix.  Mix well

Media

Unit Size

Cat. No.

Hyper Broth

500 g

2 pack

6 packa

0107-1

0107-2

0107-6

 a Six pack sold as six 500 g containers to ensure freshness during storage, prevent unintentional contamination during use, and prevent caking of powder.

 

Glucose M9Y

Cat. No. 0108

Glucose M9Y medium, used to cultivate E. coli, is a minimal medium formulation supplemented with yeast extract.  It is based on M9 salts[6] with a pH of 6.9±0.2 and employs glucose as the carbon source.  Alternative carbon sources may be substituted.  Buffering is provided by a sodium-potassium phosphate system.  Ammonium chloride provides a nitrogen source.  Yeast extract is added to improve the protein production capacity of the medium and to permit cultivation of auxotrophic strains by supplying a source of amino acids, vitamins and minerals.  This differs from the traditional formulation of glucose M9, which employs casamino acids.  Our experience has shown that yeast extract typically gives higher yields of recombinant proteins than casamino acids.

Formula – grams per liter

6.0 g sodium phosphate dibasic                                     4 g glucose

3.0 g potassium phosphate monobasic                           5 g yeast extract

1.0 g ammonium chloride                                              11 mg calcium chloride

0.5 g sodium chloride                                                    241 mg magnesium sulfate

Method of Preparation

  1. Dissolve 15.5 grams in 980 ml deionized water.

  2. Autoclave at 121°C for 15 min.

  3. Allow to cool to below 50°C.

  4. Aseptically add 20 ml of Glucose Nutrient Solution.  Mix well.

Media

Unit Size

Cat. No.

Glucose M9Y

500 g

2 pack

6 packa

0108-1

0108-2

0108-6

 a Six pack sold as six 500 g containers to ensure freshness during storage, prevent unintentional contamination during use, and prevent caking of powder.

Turbo Prime Broth™

Cat. No. 0110

Turbo Prime Broth™ is used for the cultivation of recombinant strains of E. coli.  This medium is made without animal products.  All components are certified "animal product free."  Turbo Prime Broth™ is a derivative of Turbo Broth™ developed to improve the yield of recombinant proteins.  The carbon source is glycerol which is not metabolized into acetic acid, thus improving the pH control of the culture.  It has a rich nutrient base which provides amino acids, vitamins, inorganic and trace minerals at levels higher than those of LB Broth.  The medium is buffered at pH 7.2±0.2 with potassium phosphate to prevent a drop in pH and to provide a source of phosphate.  These modifications permit higher cell yields than can be achieved with LB.  For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth.  Cell yields are typically 4 to 5 times that of LB Broth in shake flask cultures depending on the characteristics of the strain.  Plasmid copy numbers are higher, which can also enhance protein expression.  This medium is suitable as the base for fermentation cultures.  Biomass yields will depend on the process employed and the strain.  A typical bench-scale fermentation operated in a batch mode can achieve an OD600 of 10 to 12 depending on the strain.

Formula – Proprietary