GENTAUR

Home
Contents
Ask GENTAUR
Meet Us
Distributors
Google
Contact Gentaur

Promotions
GENTAUR Products
Distributed Products
Equipment
Custom Service
Order Online

info@gentaur.com

Up

GENTAUR Survey

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45
Fax 0032 16 50 90 45
info@gentaur.com

GENTAUR Ltd. GB111298832
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com
Natwest bank Whetstone
Account no. 82577846 sort code 602336

GENTAUR France SARL FR63484237888
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
RCS Paris B 484 237 888
SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com

GENTAUR GmbH
DE815175831
Marienbongard 20
52062 Aachen Deutschland
Tel tech support 0241 56 00 99 68  Frau Karolina Eland
de@gentaur.com
or  Tel 0241 95 78 94 78 Mr Tryan Ilkov
Tel logistics 0241 40 08 90 86 Annick Verdeyen
Fax 0241 55  91 05 36
Bankleitzahl 39050000
IBAN DE8839050000107569353
Handelsregister Aachen HR B 16058
Steuernummer 201/5961/3925
de@gentaur.com

GENTAUR Nederland BV NL850396268B01
Kuiper 1
5521 DG Eersel Nederland
KVK nummer 52327027
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

GENTAUR Poland Sp. z o.o. PL2040002710
Ulica Ogarna 15/19B m2
80-826 GDANSK Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              
(TEL production Warsaw 0223 98 82 21)
poland@gentaur.com

GENTAUR USA
 Genprice Inc.
Tel (408)350 0488
Tel (718)513-2983 before 9 a.m. for shipping questions
Fax (408)748-1826
3333 Bowers Avenue, Suite 130
Santa Clara, CA 95054
sales@genprice.com

GENTAUR Italy
20135 Milano
Tel 02 36 00 65 93 Fax 02 36 00 65 94
italia@gentaur.com

GENTAUR Spain
tel:0911876558
spain@gentaur.com

GENTAUR BULGARIA BG201358931
53, Graf Ignatiev Str. ent.V, fl. 2
Sofia 1000
Tel 0035929830070
Fax 0035929830072
sofia@gentaur.com

ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035929830070 Fax 0035929830072
e-mail: Sofia@gentaur.com
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

Other countries
Österreich +43720880899
Canada Montreal +15149077481
Ceská republika Praha +420246019719
Danmark +4569918806
Finland Helsset +358942419041
Magyarország Budapest +3619980547
Ireland Dublin+35316526556
Luxembourg+35220880274
Norge Oslo+4721031366
Sverige Stockholm+46852503438
Schweiz Züri+41435006251
US New York+17185132983

 

Total RNA Purification Kit
Name:
Total RNA Purification Kit
Type:
110210
Message:
Total RNA Purification Kit provides a rapid, safe and reliable way to purify total RNA from a wide variety of cells and tissues. The purified total RNA is free from contaminants, essentially is of full length and suitable for all applications of RNA.

Features
No phenol/chloroform extraction, no use of ethidium bromide and no CsCldensity gradient ultracentrifugation.
Extracted RNA is free from contaminants, easy to be redissolved in water or Tris buffer.
RNases in cells are rapidly inactivated.
High recovery of pure total RNA.

Principle
Buffer R-A containing guanidine iso-thiocyanate, a strong protein denaturant, lyses cells and releases total RNA into the supernatant. Meanwhile, Buffer R-A inactivates cellular RNases immediately to protect RNA from degradation. Addition of Buffer R-B to homogenate results in denaturation of protein, which forms insoluble clumps with genomic DNA. Proteins are further precipitated by the unique two-phase partition formed by addition of Buffer R-C. In this two-phase system RNA remains soluble and is separated into the lower phase. RNA is selectively precipitated when second two-phase partition is performed by addition of Buffer R-D into the lower phase. However, free genomic DNA remains soluble in lower phase and is separated from RNA when RNA is precipitated by centrifugation. Trace impurity is removed by second RNA precipitation performed in two-phase partition with Buffer R-E and Buffer R-F.

Procedure
Sample is homogenized in Buffer R-A. Add Buffer R-B to precipitate proteins. After addition of Buffer R-C, solution turns in to two phases and proteins are co-precipitated with genomic DNA and pelleted on the interface of the two phases and total RNA remains soluble and enters into the lower phase. Transfer the lower phase to a new microfuge tube and add Buffer R-D to form second two-phase system. In this step RNA is selectively precipitated from the lower phase, but sheared free genomic DNA, mitochondria l DNA, viral DNA or transfected vectors remain in the lower phase and are removed. The total RNA pellet is redissolved in Buffer R-A. Add buffer R-E and Buffer R-F for additional RNA precipitation wit h two-phase partition. The RNA pellet is then dissolved in H2O or Tris buffer and can be used for all RNA applications.

Applications
After first precipitation by Buffer R-D, the purified total RNA is suitable for RNA analysis experiments as follows:

Northern, dot or slot blotting
RNA protection detection
In vitro translation
DNA microarray-based analysis
Poly A+ RNA selection

After re-precipitated by Buffer R-F, the purified total RNA can be used for any RNA applications including RT-PCR and cDNA library construction.
Standard:
Cat. No. ------------------110210-04-----------------------------110210-08
Sample size-------4X108 cells/4 g tissues------------8X108 cells/8 g tissues
 
Parallel Genomic DNA & RNA Purification Kit
Name:
Parallel Genomic DNA & RNA Purification Kit
Type:
110250
Message:
Parallel Genomic DNA & RNA Purification Kit provides a rapid way to obtain pure RNA and genomic DNA from the same sample.

Features
No phenol/chloroform extraction, no CsCl density gradient ultracentrifugation.
Isolate pure RNA and DNA from same sample.

Principle and Procedure
The total RNA is released and isolated with two-phase partition as described in Total RNA Purification Kit. Genomic DNA remaining in the lower phase and precipitated on the interface of two phases is dissolved in Buffer G-A and selectively binds to silica membrane. Impurities are removed by washing with Buffer W1 and Buffer W2. Pure DNA is eluted in small volume of water or low-salt Tris buffer.

Applications
The total RNA is suitable for Northern blotting, RNA protection detection, in vitro translation, RT-PCR, cDNA library construction, etc. The genomic DNA can be applied in Southern blotting, PCR, RAPD, AFLP or RFLP, apoptosis analysis, etc.
Standard:
Cat. No. -----------------------------------110250-25-------------------------110250-50
Sample size--------------------2.5X108 cells/2.5 g tissue----------5X108 cells/5 g tissue
 

[ Home ] [ Up ]

Send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Site powered by Acid Dragon (AC)
Last modified: 07/15/09