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Diazepam ELISA Test Kit
Brief
Principle
This test kit is based on the competitive enzyme immunoassay for the detection
of Diazepam in the feed,urine, liver, meat. The coupling antigen is pre-coated
on the micro-well stripes. The Diazepam in the sample and the coupling antigen
pre-coated on the micro-well stripes compete for the anti-Diazepam antibodies.
After the addition of the enzyme conjugate, the TMB substrate is added for
coloration. The optical density (OD) value of the sample has a negative
correlation with the Diazepam in it. This value is compared to the standard
curve and the Diazepam concentration is subsequently obtained.
Specifications
Specification: 96wells /kit
Sensitivity: 0.1 ppb
Detection limit
Tissue 1 ppb
Urine 1 ppb
Feed 10 ppb
Recovery rate
Urine 80 ±10%
Feed 75 ±10%
Tissue(meat, liver) 85 ±10%
Cross-reactions rate
Diazepam 100%
Nitrazepam 7.6%
Oxazepam 8.8%