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Chloramphenicol ELISA Kit
Brief
This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the tissue (chicken,pork), honey, milk, fish, shrimp and egg. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against Chloramphenicol. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Chloramphenicol in it. This value is compared to the standard curve and the content of the corresponding Chloramphenicol is subsequently obtained.
Specifications
Sensitivity : 0.05ppb
Detection limit:
Egg, intestine 0.1 ppb
Honey 0.15ppb
Milk 0.05 ppb
Urine and serum 0.1 ppb
Chicken, pork, fish, shrimp 0.05 ppb
Recovery rate
Chicken, pork, fish, shrimp 80%±10%
Honey 70%±10%
Milk 85%±15%
Cross-reaction rate:
Chloramphenicol 100%
Thiamphenicol <0.1%
Florfeniol <0.1%