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Custom peptide polyclonal antisera have become an instrument of paramount importance in the life science tool-box. However, raising peptide polyclonal antibodies is a laborious process and for a successful outcome, numerous pitfalls have to be avoided.
Gentaur offers a complete set of
services, from peptide design to affinity purification. Whatever your
requirements are, we are confident that we will be able to accommodate you.
Though every project is different, we have a standard custom peptide polyclonal
antiserum package. This package can be extended according to your specifications
and you will also be able to make modifications and additions during the course
of a project.
The standard package includes:
Peptide Design
Peptide Synthesis, 25 mg
Peptide-Carrier Conjugation
12 week immunisation protocol for 1 rabbit according to:
Day 1 Collection of pre-immune serum
Day 1 Primary immunisation
Day 14 First booster
Day 28 Second booster
Day 42 First serum sample ~10 ml
Day 49 Third booster
Day 63 Second serum sample ~10 ml
Day 70 Fourth booster
Day 84 Production lot ~40 mlELISA analysis of the first serum sample.
Documentation of the project.
1200 EUR
Additional services are available such as:
Continuation of project
180 EUR per four-week cycleAdditional ELISA analyses
120 EUR per analysisAffinity Purification of antisera
735 EURSupplying of antiserum in aliquots
60 EUR per serum sample