Code:  24292                                                                 Price: 100 ug /350 Euro

Works in WB, not tested yet in IHC

http://au.expasy.org/cgi-bin/niceprot.pl?P50452

Entry information

Entry name SPB8_HUMAN Primary accession number P50452 Secondary accession numbers None Entered in Swiss-Prot in Release 34, October 1996 Sequence was last modified in Release 34, October 1996 Annotations were last modified in    Release 44, July 2004 Name and origin of the protein Protein name Cytoplasmic antiproteinase 2 Synonyms CAP2 CAP-2 Protease inhibitor 8 Serpin B8 Gene name   Name:  SERPINB8   Synonyms:  PI8 From Homo sapiens (Human)  [TaxID: 9606] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.

References

[1]   NUCLEOTIDE SEQUENCE. TISSUE=Placenta; DOI=10.1074/jbc.270.50.29854;MEDLINE=96102039;PubMed=8530382 [NCBI, ExPASy, EBI, Israel, Japan] Sprecher C.A., Morgenstern K.A., Mathewes S., Dahlen J.R., Schrader S.K., Foster D.C., Kisiel W.; "Molecular cloning, expression, and partial characterization of two novel members of the ovalbumin family of serine proteinase inhibitors."; J. Biol. Chem. 270:29854-29861(1995).

Comments

SUBCELLULAR LOCATION: Cytoplasmic. SIMILARITY: Belongs to the serpin family. Ov-serpin subfamily.

Copyright

This Swiss-Prot entry is copyright. It is produced through a collaboration between the Swiss Institute of Bioinformatics and the EMBL outstation - the European Bioinformatics Institute. There are no restrictions on its use as long as its content is in no way modified and this statement is not removed.

Cross-references

EMBL L40377; AAC41939.1; -. [EMBL / GenBank / DDBJ] [CoDingSequence] PIR A59273; A59273. HSSP P05120; 1BY7. [HSSP ENTRY / PDB] Ensembl ENSG00000166401; Homo sapiens. [Contig view] Genew HGNC:8952; SERPINB8. CleanEx HGNC:8952; SERPINB8. GeneCards SERPINB8. GeneLynx SERPINB8; Homo sapiens. GenAtlas SERPINB8. MIM 601697 [NCBI / EBI]. GO GO:0005829; Cellular component: cytosol (traceable author statement). GO:0005515; Molecular function: protein binding (traceable author statement). GO:0004867; Molecular function: serine-type endopeptidase inhibitor activity (traceable author statement). QuickGo view. SOURCE SERPINB8; Homo sapiens. InterPro IPR000215; Prot_inh_serpin. Graphical view of domain structure. Pfam PF00079; Serpin; 1. Pfam graphical view of domain structure. SMART SM00093; SERPIN; 1. PROSITE PS00284; SERPIN; 1. ProDom [Domain structure / List of seq. sharing at least 1 domain] HOVERGEN [Family / Alignment / Tree] BLOCKS P50452. ProtoNet P50452. ProtoMap P50452. PRESAGE P50452. DIP P50452. ModBase P50452. SMR P50452; E855A033519AC60E. SWISS-2DPAGE Get region on 2D PAGE. UniRef View cluster of proteins with at least 50% / 90% identity.

Keywords

Serine protease inhibitor; Serpin.

Features

Feature table viewer Key From   To  Length   Description   SITE    339   340   2       Reactive bond (By similarity).

Sequence information

Length: 374 AA Molecular weight: 42786 Da CRC64: E855A033519AC60E [This is a checksum on the sequence]

10 20 30 40 50 60 MDDLCEANGT FAISLFKILG EEDNSRNVFF SPMSISSALA MVFMGAKGST AAQMSQALCL 70 80 90 100 110 120 YKDGDIHRGF QSLLSEVNRT GTQYLLRTAN RLFGEKTCDF LPDFKEYCQK FYQAELEELS 130 140 150 160 170 180 FAEDTEECRK HINDWVAEKT EGKISEVLDA GTVDPLTKLV LVNAIYFKGK WNEQFDRKYT 190 200 210 220 230 240 RGMLFKTNEE KKTVQMMFKE AKFKMGYADE VHTQVLELPY VEEELSMVIL LPDDNTDLAV 250 260 270 280 290 300 VEKALTYEKF KAWTNSEKLT KSKVQVFLPR LKLEESYDLE PFLRRLGMID AFDEAKADFS 310 320 330 340 350 360 GMSTEKNVPL SKVAHKCFVE VNEEGTEAAA ATAVVRNSRC SRMEPRFCAD HPFLFFIRRH 370 KTNCILFCGR FSSP

LOCUS       NP_942130                374 aa            linear   PRI 27-OCT-2004
DEFINITION  serine (or cysteine) proteinase inhibitor, clade B (ovalbumin),
            member 8 [Homo sapiens].
ACCESSION   NP_942130
VERSION     NP_942130.1  GI:38504669
DBSOURCE    REFSEQ: accession NM_198833.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 374)
  AUTHORS   Reddy,P.H., Stockburger,E., Gillevet,P. and Tagle,D.A.
  TITLE     Mapping and characterization of novel (CAG)n repeat cDNAs from
            adult human brain derived by the oligo capture method
  JOURNAL   Genomics 46 (2), 174-182 (1997)
   PUBMED   9417904
REFERENCE   2  (residues 1 to 374)
  AUTHORS   Bartuski,A.J., Kamachi,Y., Schick,C., Overhauser,J. and
            Silverman,G.A.
  TITLE     Cytoplasmic antiproteinase 2 (PI8) and bomapin (PI10) map to the
            serpin cluster at 18q21.3
  JOURNAL   Genomics 43 (3), 321-328 (1997)
   PUBMED   9268635
REFERENCE   3  (residues 1 to 374)
  AUTHORS   Sprecher,C.A., Morgenstern,K.A., Mathewes,S., Dahlen,J.R.,
            Schrader,S.K., Foster,D.C. and Kisiel,W.
  TITLE     Molecular cloning, expression, and partial characterization of two
            novel members of the ovalbumin family of serine proteinase
            inhibitors
  JOURNAL   J. Biol. Chem. 270 (50), 29854-29861 (1995)
   PUBMED   8530382
COMMENT     VALIDATED REFSEQ: This record has undergone preliminary review of
            the sequence, but has not yet been subject to final review. The
            reference sequence was derived from BX280409.1, AL708543.1,
            BX571754.1, L40377.1, BG611734.1, BE566411.1 and CA445967.1.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.3"
     Protein         1..374
                     /product="serine (or cysteine) proteinase inhibitor, clade
                     B (ovalbumin), member 8"
                     /note="protease inhibitor 8 (ovalbumin type); cytoplasmic
                     antiproteinase 2"
     CDS             1..374
                     /gene="SERPINB8"
                     /coded_by="NM_198833.1:170..1294"
                     /note="go_component: cytosol [goid 0005829] [evidence TAS]
                     [pmid 8530382];
                     go_function: protein binding [goid 0005515] [evidence TAS]
                     [pmid 8530382];
                     go_function: serine-type endopeptidase inhibitor activity
                     [goid 0004867] [evidence IEA];
                     go_function: serine-type endopeptidase inhibitor activity
                     [goid 0004867] [evidence TAS] [pmid 8530382]"
                     /db_xref="GeneID:5271"
                     /db_xref="MIM:601697"
ORIGIN      
        1 mddlceangt faislfkilg eednsrnvff spmsissala mvfmgakgst aaqmsqalcl
       61 ykdgdihrgf qsllsevnrt gtqyllrtan rlfgektcdf lpdfkeycqk fyqaeleels
      121 faedteecrk hindwvaekt egkisevlda gtvdpltklv lvnaiyfkgk wneqfdrkyt
      181 rgmlfktnee kktvqmmfke akfkmgyade vhtqvlelpy veeelsmvil lpddntdlav
      241 vekaltyekf kawtnseklt kskvqvflpr lkleesydle pflrrlgmid afdeakadfs
      301 gmsteknvpl skvahkcfve vneegteaaa atavvrnsrc srmeprfcad hpflffirhh
      361 ktncilfcgr fssp
//