Anti-MAP2

Product Name

MAP2 antibody - Neuronal Marker

Product type

Primary antibodies

Description

Rabbit polyclonal to MAP2 - Neuronal Marker

Immunogen

Cow brain MAP 2 purified from the material that pellets with GTP polymerized tubulin (see references).

Cross Reactivity

Cross-reacts with Cow, Human, Mouse and Rat.
Not yet tested in other species.

Tested applications

Western blot, Immunofluorescence

Application notes

IF: Use at a dilution of 1/30000.
WB: Use at a dilution of 1/300000. Detects a band of approximately 280 kDa, corresponding to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localisation

Cytoplasmic

Research areas

Neuroscience, Cytoskeleton

Relevance

Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one alpha subunit and one beta tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins, which includes the protein t and MAP 1, MAP 2, MAP 3, MAP 4 and MAP 5. MAP 2 is found only in neuronal cells and specifically in the perikarya and dendrites. Antibodies to MAP2 are therefore excellent markers of neuronal cells and neuronal dendrites.

Alternative names for MAP2 antibody - Neuronal Marker (ab5392)

Database links

GeneCard		GC02P209169 (Human)
Locus Link		4133 (Human) 17756 (Mouse) 25595 (Rat)
Omim		157130 (Human)

SwissProt		P11137 (Human) P20357 (Mouse) P15146 (Rat)
Unigene		167 (Human) 256966 (Mouse) 10484 (Rat)

Raised in

Rabbit

Clonality

Polyclonal

Isotype

IgG

Storage buffer

Preservative: 5mM Sodium Azide
Constituents: PBS, Whole Serum

Purification notes

Lipid extraction from egg yolk, followed by ammonium sulphate precipitation.

Form

Liquid, 200 µl 325 Euro

Concentration

19.00 mg/ml

Storage instructions

Aliquot and store at -20°C or -80°C.
Avoid repeated freeze / thaw cycles.

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Send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Site powered by Acid Dragon (AC)
Last modified: 07/15/09