Annexin V

TACS^TM Annexin V Kits

Annexin V protocol
Annexin V brochure

TACS Annexin V Kits allow rapid, specific, and quantitative identification of apoptosis in individual cells. Annexin V is a calcium-dependent phospholipid binding protein. During apoptosis, an early and ubiquitous event is the exposure of phosphatidylserine at the cell surface. Trevigen offers annexin V conjugated to either FITC or to biotin for the detection of cell surface changes during apoptosis. The annexin V conjugates are supplied with an optimized Binding Buffer and propidium iodide. Propidium iodide may be used on unfixed samples to determine the population of cells that have lost membrane integrity, an indication of late apoptosis or necrosis.

Features:	Fast, sensitive detection of apoptotic cells. Includes propidium iodide for discriminating apoptotic and necrotic (or late apoptotic) cells. Annexin V-Biotin provides flexibility in fluorophore choice.
Applications:	Flow cytometry In situ detection of apoptotic cells
	Annexin V FITC Kits
Components:	Component	Size	Price
	Annexin V FITC (A)	100 µl	323 €
	10X Binding Buffer (A)	8 ml	84 €
	Propidium Iodide (A)	1 ml	84 €
	Annexin V FITC (B)	250 µl	665 €
	10X Binding Buffer (B)	20 ml	103 €
	Propidium Iodide (B)	2.5 ml	103 €

	(A) 4830-01-K Annexin V FITC Kit 100 Samples 347 € (B) 4830-250-K Annexin V FITC Kit 250 Samples 690 €

	Annexin V Biotin Kits
Components:	Component	Size	Price
	10X Binding Buffer (A)	8 ml	84 €
	Propidium Iodide (A)	1 ml	84 €
	10X Binding Buffer (B)	20 ml	103 €
	Propidium Iodide (B)	2.5 ml	103 €
	Annexin V Biotin (A)	100 µl	371 €
	Annexin V Biotin (B)	250 µl	694 €

	(A) 4835-01-K Annexin V Biotin Kit 100 Samples 381 € (B) 4835-250-K Annexin V Biotin Kit 250 Samples 708 €

Storage:	Stable for at least one year when stored at 4°C.

References:	1. Fadock, V.A., D.R. Voelker, P.A .Campbell, J.J. Cohen, D.L. Bratton, and P.M. Henson. 1992. Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J Immunol 148:2207-2216. 2. Tait, J.F., and D. Gibson. 1992. Phospholipid binding of Annexin V: Effects of calcium and membrane phosphatidylserine content. Arch Biochemiand Biophys 298(1):187-191. 3. Meers, P., and T. Mealy. 1993. Calcium-dependent Annexin V binding to phospholipids: stoichiometry, specificity, and the role of negative charge. Biochemistry 32:11711-11721.

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Send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Site powered by Acid Dragon (AC)
Last modified: 07/15/09