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Agarose LE
(Low Electroendosmosis)
Agarose LE from Labnet is a high quality molecular biology grade Agarose
suitable for analytical and preparative electrophoresis of nucleic acids.
Nucleic acid separation with Agarose LE is between 0.2–15 kbp depending on the
concentration of Agarose LE.
Applications
Nucleic acid fragments separated with Agarose LE can be blotted to nylon or
nitro-cellulose membranes by all standard blotting techniques.
Detection with non-radioactive probes, e.g. digoxigenin (DIG) - labeled nucleic
acids, does not interfere with the use of Agarose LE.
LE is tested for preparative electrophoresis and isolation of DNA fragments.
However, we recommend also our low melting point Agarose LM that is optimized
for these applications.
Specifications
Clarity 1.5% (NTU)
≤ 3%
Electroendosmosis (EEO)
0.05 - 0.13
Sulfur as SO4
0.14%
Gelling temperature (1.5 %)
36° C (±_1.5°C)
Melting temperature (1.5 %)
88° C (±_1.5°C)
Gel strength (1%)
1200 g/cm2
Gel strength (1.5 %)
2500 g/cm2
DNase
none detected
RNase
none detected
Storage and Stability
Store in a cool dry place at 15-25°C.
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Protocol:
Electrophoresis of DNA and RNA
The most commonly used technique for DNA separation is electrophoresis in
horizontal agarose gels submerged in either Tris-acetate or Tris-borate buffer.
RNA molecules are separated in denaturing agarose
gels containing formaldehyde. RNA gels are submerged in MOPS buffer.
Step1:
Add Agarose LE to a measured volume of
buffer in a beaker or flask that is 3 times the volume of solution prepared. The
amount of Agarose LE added depends on the desired concentration.
Step2: Dissolve the
Agarose by melting, simply by heating the slurry in a boiling water bath. If you
are using a microwave oven, heat
on high power and mix gently.
Step3: Cool the
solution to approx. 50°C before pouring.
Efficient separation of DNA fragments of a wide size range can be achieved by
adjusting Agarose LE concentration (see table).
Results
Agarose LE gels in IX TAE buffer A-0.75%, B-1%, and C-1.25%ffer A-0.75%, B-1%, and C-1.25%
Marker: 1kb ladder.
Electrophoresis
conditions: submarine gel,
2 hours 30 min,
4.5 V/cm in 1XTAE
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